Leica Microsystems DMC4500 Application Note

DMC4500
Leica Microsystems
ブランド
Leica Microsystems
モデル
DMC4500
タイプ
Application Note

このマニュアルも適しています

From Eye to Insight
James DeRose, Ph.D.
Scientific Writer, Applied Microscopy Marketing,
Leica Microsystems AG, Switzerland
Martin Gamerdinger, Ph.D.
Scientific Project Leader, Molecular Microbiology,
University of Constance, Germany
Heinrich Bürgers, Ph.D.
Product Manager, Life Science Research Stereo Microscope,
Leica Microsystems AG, Switzerland
2
RNA
/
Caenorhabditis elegans
40
[1] [2]
[3]
302
1/3
15 20
°
C 1 mm 75 µm
1
DNA
/
/
RNA
RNAi
dsRNA siRNA
microRNA
RNAi
/ /
3
1
1.
[4]
2. a)
[5 6]
2. b)
RNA
RNAi [7] 2
ds RNA
3.
RNAi
4.
5.
2
•
→
•
→
•
→
[4]
•
→
•
→
S/N
•
60
p. 2
2
4
3
3
S6 [8]
LED 2500 [9] 4 S6
10
16
3
LED2500 S6 [8]
[9] 1.6
S6
5 S6 4 S6 LED2500
5
LED2500 S6
LED 2500
•
•
•
•
LED
•
•
•
•
6 M80 TL3000 ST
M80 [10] TL3000 ST [11]
6 M80 CMO
500 MC 170/190 HD [12] HD
S6 LED2500
6
RNAi
/
2 [13] CRISPR/Cas9 [14]
CRISPR 1
[6]
5
DNA
DIC [15] IMC [16]
DMi8 7 DM IL LED [17]
Narishige Instruments 3
RNA RNAi
2 ds RNA
dsRNA
si RNA
siRNA
7
DMi8
7
20
°
C
2
5 [18]
10
[19]
GFP
[20]
DsRed [20]
GFP
Rottermann contrast
[FOV]
M165 FC [21] MZ10 F [22]
TL4000 RC [11]
8
•
M165 FC TL4000
•
M165 FC MZ10 F TL4000
•
TripleBeam
S/N
[23]
→
8 M165 FC TL4000 RC Rottermann contrast
8
YouTube
[24] 1
mCherry [20] M205 FA
DMC4500
•
•
•
TL4000 RCI [11]
•
TL4000 RC
•
S/N
•
TL3000 ST TL4000 BFDF [11] TL4000 RC
9
TCS SP8
TCS SPE [25] DM6 DM2500 [26]
GFP RFP
mCherry DsRed YFP CFP [20] 9 10
Levamisol
[27]
9 GFP TCS SP8
10
10 SP2 CFP GFP YFP
DsRed DiD BC
H. Hutter [28]
[29] / /
•
S6 TL3000 ST
•
DMi8
DNA
•
MZ10 F M165 FC
TL4000 RC
•
TL4000 RC CMO
M80
•
DM6 TCS SP8
•
M205 FA TL5000 Ergo [11]
•
•
11
Harald Hutter BC
1.
S. Brenner
Nature s Gift to Science Nobel Prize Lecture (December, 2002) The Nobel Foundation
2.
The
C. elegans
Sequencing Consortium Genome Sequence of the Nematode
C. elegans
: A Platform for Investigating
Biology
Science, vol. 282 (1998)
3.
S.W. Emmons
The beginning of connectomics: a commentary on White et al. (1986) The structure of the nervous
system of the nematode
Caenorhabditis elegans
Philosophical Transactions of the Royal Society B, vol. 370, iss. 1666
(2015), DOI: 10.1098/rstb.2014.0309
4.
T. Stiernagle
Maintenance of
C. elegans
Wormbook: The Online Review of
C. elegans
Biology (2006)
5.
P.J. Schweinsberg, B.D. Grant
C. elegans
gene transformation by microparticle bombardment Wormbook: The Online
Review of
C. elegans
Biology (2013)
6.
T.C. Evans
Transformation and microinjection Wormbook: The Online Review of
C. elegans
Biology (2006)
7.
J. Ahringer
Reverse genetics Wormbook: The Online Review of
C. elegans
Biology (2006)
8.
S6
9.
10.
M50 M60 M80
11.
12.
MC170 HD
13.
L.A. Pray
Transposons: The jumping genes Nature Education, vol. 1 iss. 1, p. 204 (2008),
14.
F.A. Ran, P.D. Hsu, J. Wright, V. Agarwala, D.A. Scott, F. Zhang
Genome engineering using the CRISPR-Cas9 system
Nature Protocols, vol. 8, pp. 2281–2308 (2013), doi:10.1038/nprot.2013.143
15.
W. Ockenga
Differential Interference Contrast (DIC) DIC
16.
B. Kleine, T. Veitinger
Integrated Modulation Contrast (IMC): Oblique Illumination Enhances Visibility of Living Cells
IMC
17.
18.
T. Mahmood, P.-C. Yang
Western Blot: Technique, Theory, and Trouble Shooting N. Am. J. Med. Sci. vol. 4, iss. 9,
pp. 429–434 (2012), doi: 10.4103/1947-2714.100998
19.
T. Mašek, L. Valášek, M. Pospíšek
Polysome analysis and RNA purification from sucrose gradients Methods Mol.
Biol. vol. 703, pp. 293-309 (2011), doi: 10.1007/978-1-59745-248-9_20
20.
C. Greb
Fluorescent Proteins – Introduction and Photo Spectral Characteristics
21.
M165 FC M205 FA
22.
MZ10 F
23.
B. Fuchs
Stereo microscopes with TripleBeam Technology: Third illumination path for better signal-to-noise ratio in
fluorescence microscopy
TripleBeam SN 3
24.
Z. Pincus, T.C. Mazer, F.J. Slack
Autofluorescence as a measure of senescence in
C. elegans
: look to red, not blue or
green
Aging, vol. 8, no. 5, pp. 889-898 (2016)
25.
26.
27.
28.
H. Hutter
Five-colour in vivo imaging of neurons in
Caenorhabditis elegans
Journal of Microscopy, vol. 215, pt. 2, pp.
213 –218 (2004), doi: 10.1111/j.0022-2720.2004.01367.x
29.
Worm Book: The Online Review of
C. elegans
Biology
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Leica Microsystems DMC4500 Application Note

DMC4500
Leica Microsystems
ブランド
Leica Microsystems
モデル
DMC4500
タイプ
Application Note
このマニュアルも適しています
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