Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

LOH

(Loss of Heterozygosity)

LOH

(Loss of Heterozygosity)

GeneMapper® v4.0

LOH

Getting Started Guide

GeneMapper® v4.0

LOH

Getting Started Guide

LOH

(Loss of Heterozygosity)

LOH

(Loss of Heterozygosity)

ii GeneMapper® Software v4.0 LOH Getting Started Guide

Applied Biosystems

:

Applera Corporation

GeneMapper HID

:

ABI Prism Applied Biosystems GeneMapper GeneScan SNaPshot AB Design Applera GeneMapper GeneScan SNPlex ZipChute

Applera Corporation

AFLP Keygene N.V

Microsoft Windows Microsoft Corporation

Oracle Oracle Corporation

Applera Corporation is committed to providing the world’s leading technology and information for life scientists. Applera Corporation consists of the Applied

Biosystems and Celera Genomics businesses.

Part Number 4363081 Rev. B

06/2005

GeneMapper® Software v4.0 LOH Getting Started Guide iii

v

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

11

LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

GeneMapper® Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

GeneMapper v3.7 . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2LOH (Loss of Heterozygosity) 7

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Kit Panel Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . 14

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Auto Bin Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

331

Analysis Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4 LOH (Loss of Heterozygosity) 39

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

LOH Report Setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

iv GeneMapper® Software v4.0 LOH Getting Started Guide

551

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

55

GeneMapper® Software v4.0 LOH Getting Started Guide v

GeneMapper® Software v4.0 LOH Getting Started Guide

Applied Biosystems Data Collection

LOH

GeneMapper

Software

GeneMapper Software

•GeneMapper® Software v4.0 Quick Installation and Administration Guide (PN 4363080)

GeneMapper Software v4.0

• Microsoft® Winows® XP

•

0 [Enter]

•

•

File Open Spot Set

View Filter View All Runs

Applied Biosystems Getting Started Guide 2

vi GeneMapper® Software v4.0 LOH Getting Started Guide

Control Console

Oracle

ID

1 96 1 Sample Entry

GeneMapper® Software v4.0 Installation and Administration Guide (PN 4363080)

GeneMapper® Software v4.0 Installation and

Administration Guide (PN 4363080)

GeneMapper® Software 4.0

Installation and Administration Guide (PN 4363080)

•GeneMapper® Software v4.0 Installation and Administration Guide – GeneMapper

Software v4.0

•GeneMapper® Software v4.0 Getting Started Guide – GeneMapper Software

5

Applied Biosystems Data

Collection LOH AFLP®

SNaPshot® SNPlex™

GeneMapper Software

•GeneMapper® Software v4.0 Online Help –

GeneMapper Software

[F1]

Help Contents and Index GeneMapper

3

•GeneMapper® Software v4.0 Quick Reference Guide –

GeneMapper Software

•GeneMapper® Software v4.0 Reference and Troubleshooting Guide –

GeneMapper® Software v4.0 LOH Getting Started Guide vii

PDF

GeneMapper Software v4.0 Documentation CD

vii

GeneMapper Software

[F1]

Help Contents and Index GeneMapper

3

Applied Biosystems

jptechsupport@appliedbiosystems.com

http://www.appliedbiosystems.co.jp Customer Support

•FAQ

•

• Applied Biosystems MSDS Certification of Analysis

•PDF

•

Applied Biosystems

viii GeneMapper® Software v4.0 LOH Getting Started Guide

1

L

O

H

(

Loss of Heterozygosity)

L

O

H

(Loss of Heterozy

g

osity

)

GeneMapper® Software v4.0 LOH Getting Started Guide 1

■LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

■LOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

■GeneMapper® Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

■ . . . . . . . . . . . . . . . . . . 5

■GeneMapper v3.7 . . . . . . . . . . . . . . . . . 5

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1

LOH

2GeneMapper® Software v4.0 LOH Getting Started Guide

LOH

STR

DNA 1

2 7 1

PCR PCR

GeneMapper

LOH TSG two-hit

TSG

2 TSG

LOH

Loss of Heterozygosity; TSG

1

LOH LOH LOH

LOH TSG DNA

LOH

2 LOH

2 1

1-1

DNA

1-1 2

GeneMapper® Report Manager

Peak Height Ratio

Peak-Height Ratio LOH

DNA

Applied Biosystems

Applied Biosystems Web www.appliedbiosystems.co.jp

1

2

1

2

1

GeneMapper® Software v4.0 LOH Getting Started Guide 3

LOH Applied Biosystems

GeneMapper® Software v4.0 Quick Reference Guide (PN 4362816)

GeneMapper

®

LOH Default Report Setting

: 1_Healthy.fsa, 1_Tumor.fsa, 2_Healthy.fsa, 2_Tumor.fsa

LOH LOH Default Report Setting

LOH GeneMapper®

LOH 45

LOH Report Setting Report Setting

: Healthy_1.fsa, Healthy_2.fsa, …,Tumor_2.fsa, Tumor_1.fsa

4 .fsa

<drive>:\AppliedBiosystems\GeneMapper\Example Data\LOH

GeneMapper®

D

PCR

GeneScan

™

500LIZ

®

ABI PRISM

®

3100

6

2LOH (Loss of Heterozygosity)

Panel Marker

bp

R5 Blue 120 – 186

R26 Blue 193 – 295

R14 Blue 300 – 470

R21 Yellow 100 – 160

R24 Yellow 170 – 250

R2 Green 157 – 204

1

LOH

4GeneMapper® Software v4.0 LOH Getting Started Guide

LOH

GeneMapper® LOH

(5)

1.

2.

LOH (Loss of Heterozygosity)

(2)

1. Kit Panel Marker

2.

3. Table Settings

4.

5. Bin Set Bin Auto Bin

(3)

1. Analysis Method Bin Set

2.

3.

LOH (Loss of Heterozygosity)

(4)

1. Genotypes LOH

2. LOH

3. LOH Report Setting

1

GeneMapper® Software

GeneMapper® Software v4.0 LOH Getting Started Guide 5

GeneMapper® Software

GeneMapper®

1. Start All Programs Applied Biosystems GeneMapper 

GeneMapper 4.0

2. Login to GeneMapper

a.

b. OK

LOH

GeneMapper® Software Online Help

GeneMapper v3.7

LOH

Analysis Method Size Standard Panel

GeneMapper®

Bin

Marker bp Bin

Marker

Bin Set

Bin ) Bin Set

Marker

bp

Panel

Marker GeneMapper® Panel Bin Set

Marker Bin

Kit

Panel

1

6GeneMapper® Software v4.0 LOH Getting Started Guide

GeneMapper® LOH

GeneMapper

LOH 2LOH

(Loss of Heterozygosity) LOH

GeneMapper

Data Collection

LOH

GeneMapper® Software v4.0 Quick Installation

and Administration Guide (PN 4363080)

GeneMapper

2

LOH (Loss of Heterozygosity)

LOH

GeneMapper

GeneMapper® Software v4.0 Quick Installation and

Administration Guide (PN 4363080)

2

LOH

(Loss of Heterozygosity)

LO

H

(Loss of Heterozygosity

)

GeneMapper® Software v4.0 LOH Getting Started Guide 7

LOH (Loss of Heterozygosity)

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

■Kit Panel Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

■ . . . . . . . . . . . . . . . . . . . . . . . . . . 12

■ Table Settings . . . . . . . . . . . . . . . 14

■ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

■Auto Bin Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . 25

2LOH (Loss of Heterozygosity)

8GeneMapper® Software v4.0 LOH Getting Started Guide

•KitPanel Marker

•

• Display Settings

•

• AutoBin Bin Set Bin

GeneMapper

GeneMapper® Software Online Help

• Creating a New Kit Kit

• Creating a Custom Panel

• Creating Markers Marker

• Creating a Project

• Adding Samples

• Applying Analysis Settings Analysis Settings

• Starting Analysis

• Creating a New Bin Set Bin Set

• Using the Auto Bin Function Auto Bin

Help [F1]

2LOH (Loss of Heterozygosity)

Kit Panel Marker

GeneMapper® Software v4.0 LOH Getting Started Guide 9

Kit Panel Marker

Panel Manager

•Kit – Panel

•Panel – Marker

•Marker – bp

Panel Marker Marker

Panel

Applied Biosystems Linkage Mapping Set

GeneMapper® Panel Panel

GeneMapper® Software Online Help

Kit Panel

Marker

Kit Panel Marker

1. Panel Manager Too ls Panel Manager

2. Panel Manager

File New Kit

2LOH (Loss of Heterozygosity)

Kit Panel Marker

10 GeneMapper® Software v4.0 LOH Getting Started Guide

3. New Kit Kit Name LOH Kit Kit Type

Microsatellite OK

LOH Kit

4. LOH Kit File New

Panel

5. Panel Manager New Panel Panel Name LOH Panel

[Enter]

LOH Kit LOH Panel

6. LOH Panel File New

Marker

ページが読み込まれています...

Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

関連論文

その他のドキュメント