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GeneMapper® v4.0
Getting Started Guide
GeneMapper® v4.0
Getting Started Guide
ii GeneMapper® Software Version 4.0 Getting Started Guide
© Copyright 2005, Applied Biosystems.All rights reserved.
Applied Biosystems
Applied Biosystems
:
Applera Corporation
GeneMapper HID
GeneMapper HID
:
ABI PRISM, Applied Biosystems, GeneMapper, GeneScan, LIZ and SNaPshot are registered trademarks and AB(Design), Applera GeneMapper, GeneScan and
SNPlex are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries.
This product includes software developed by the Apache Software Foundation (http://www.apache.org/). Copyright © 1999-2000
The Apache Software Foundation. All rights reserved.
This product includes software developed by the ExoLab Project (http://www. exolab.org/). Copyright 2000 © Intalio Inc. All
rights reserved.
JNIRegistry is Copyright © 1997 Timothy Gerard Endres, ICE Engineering, Inc., http://www.trustice.com.
Oracle is a registered trademark of Oracle Corporation.
All other trademarks are the sole property of their respective owners.
Applera Corporation is committed to providing the world’s leading technology and information for life scientists. Applera Corporation consists of the Applied
Biosystems and Celera Genomics businesses.
GeneMapper® Software Version 4.0 Getting Started Guide iii
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GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
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GeneMapper v3.7 . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
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Kit Panel Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
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Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . 14
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Auto Bin Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
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Analysis Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
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iv GeneMapper® Software Version 4.0 Getting Started Guide
GeneMapper® Software Version 4.0 Getting Started Guide v
GeneMapper® Software Version 4.0 Getting Started Guide
Applied Biosystems Data Collection
GeneMapper
GeneMapper
•
GeneMapper
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Software Version 4.0 Installation and Administration
Guide (PN 4363080)
GeneMapper v4.0
• Microsoft® Winows® XP
•
0 [Enter]
•
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File Open Spot Set
View Filter View All Runs
Applied Biosystems 2
Control Console
vi GeneMapper® Software Version 4.0 Getting Started Guide
Oracle
ID
1 96 1 Sample Entry
GeneMapper® Software v4.0 Installation and Administration Guide (PN 4363080)
GeneMapper® Software v4.0 Installation and
Administration Guide (PN 4363080)
GeneMapper® Software 4.0
Installation and Administration Guide (PN 4363080)
•GeneMapper® Software v4.0 Installation and Administration Guide – GeneMapper
v4.0
•GeneMapper® Software v4.0 Getting Started Guide – GeneMapper
5
Applied Biosystems Data
Collection LOH AFLP®
SNaPshot® SNPlex™
GeneMapper
•GeneMapper® Software v4.0 Online Help – GeneMapper
[F1]
Help Contents and Index GeneMapper
3
•GeneMapper® Software v4.0 Quick Reference Guide –
GeneMapper
•GeneMapper® Software v4.0 Reference and Troubleshooting Guide –
PDF
GeneMapper Software v4.0 Documentation CD
vii
GeneMapper
[F1] Help Contents and Index GeneMapper
3
viii GeneMapper® Software Version 4.0 Getting Started Guide
1
GeneMapper® Software Version 4.0 Getting Started Guide 1
■ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
■GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
■ . . . . . . . . . . . . . . . . . . 5
■GeneMapper v3.7 . . . . . . . . . . . . . . . . . 5
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1
2GeneMapper® Software Version 4.0 Getting Started Guide
STR
DNA 1
2 7 1
PCR PCR
GeneMapper®
Applied Biosystems PCR
Applied Biosystems Web www.appliedbiosystems.co.jp
Applied Biosystems
GeneMapper® Software Version 4.0 Quick Reference Guide
(PN 4362816)
1
GeneMapper® Software Version 4.0 Getting Started Guide 3
3
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GeneMapper®
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bp
D6S264 Blue 108 – 130
D6S1574 Blue 146 – 172
D6S276 Blue 201 – 233
D5S408 Blue 240 – 285
D6S308 Blue 326 – 354
D6S287 Green 104 – 138
D6S292 Green 154 – 176
D6S434 Green 201 – 245
D5S426 Green 274 – 298
D5S1981 Yellow 112 – 125
D6S257 Yellow 167 – 195
D6S446 Yellow 212 – 234
D5S641 Yellow 299 – 339
D5S433 Red 69 – 99
D5S406 Red 168 – 196
D5S400 Red 221 – 243
D6S309 Red 307 – 333
1
4GeneMapper® Software Version 4.0 Getting Started Guide
GeneMapper®
GeneMapper®
(4)
•
•
(2)
1. Kit Panel Marker
2.
3. Table Settings
4.
5. Bin Set Bin Auto Bin
(3)
1. Analysis Method Bin Set
2.
3.
Analysis Method Size Standard Panel
GeneMapper®
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Marker bp Bin
Marker
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Marker Bin
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Panel
1
GeneMapper® Software Version 4.0 Getting Started Guide 5
GeneMapper®
1. Start All Program Applied Biosystems GeneMapper GeneMapper
4.0
2. Login to GeneMapper
a.
b. OK
GeneMapper®
Software Online Help
GeneMapper v3.7
GeneMapper v3.7
GeneMapper v3.7
GeneMapper v3.7
2
GeneMapper® Software Version 4.0 Getting Started Guide 7
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
■Kit Panel Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
■ . . . . . . . . . . . . . . . . . . . . . . . . . . 12
■ Table Settings . . . . . . . . . . . . . . . 14
■ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
■Auto Bin Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . 25
2
8GeneMapper® Software Version 4.0 Getting Started Guide
•KitPanel Marker
•
• Display Settings
•
• AutoBin Bin Set Bin
GeneMapper
GeneMapper® Software Online Help
• Creating a New Kit Kit )
• Creating a Custom Panel )
• Creating Markers Marker )
• Creating a Project )
• Adding Samples )
• Applying Analysis Settings Analysis Settings )
• Starting Analysis )
• Creating a New Bin Set Bin Set )
• Using the Auto Bin Function Auto Bin )
Help [F1]
2
Kit Panel Marker
GeneMapper® Software Version 4.0 Getting Started Guide 9
Kit Panel Marker
Panel Manager
•Kit – Panel
•Panel – Marker
•Marker – bp
Panel Marker Marker
Panel Applied
Biosystems Linkage Mapping Set GeneMapper®
Panel Panel
GeneMapper® Software Online Help
Kit Panel
Marker
Kit Panel Marker
1. Panel Manager Too ls Panel Manager
2. Panel Manager
File New Kit
3. New Kit Kit Name Microsatellite Kit Kit Type
Microsatellite OK
2
Kit Panel Marker
10 GeneMapper® Software Version 4.0 Getting Started Guide
Microsatellite Kit
4. Microsatellite Kit File
New Panel
5. Panel Manager New Panel Panel Name Microsatellite
Panel Enter
Microsatellite Kit Microsatellite Panel
6. Microsatellite Panel
File New Marker
7. Panel Manager Marker
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