Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

GeneMapper® v4.0

SNaPshot

®

Kit SNP

Getting Started Guide

Panel

Bin Set

Primer Focus® Kit

Panel

Bin Set

SNaPshot® Kit

GeneMapper® v4.0

SNaPshot

®

Kit SNP

Getting Started Guide

Panel Bin Set

Primer Focus® Kit

Panel

Bin Set

SNaPshot® Kit

ii GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

Copyright

Applied Biosystems

:

Applera Corporation

GeneMapper Software has not undergone specific developmental validation for human identification applications. Human identification laboratories

analyzing single-source or parentage samples which choose to use GeneMapper Software for data analysis should perform their own developmental

validation studies.

:

ABI PRISM, Applied Biosystems, GeneMapper, LIZ, Primer Focus, and SNaPshot are registered trademarks, and the AB Design, Applera, GeneMapper and

SNPlex are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries.

This product includes software developed by the Apache Software Foundation

AFLP is a registered trademark of Keygene N.V.

Microsoft is registered trademark of Microsoft Corporation.

Oracle is a registered trademark of Oracle Corporation.

All other trademarks are the sole property of their respective owners.

Applera Corporation is committed to providing the world's leading technology and information for life scientists. Applera Corporation consists of the

Applied Biosystems and Celera Genomics businesses.

Part Number 4363078 Rev. B

06/2005

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide iii

v

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SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

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SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

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GeneMapper v3.7 . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

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2 Panel Bin Set 9

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Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

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Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3Primer Focus® Kit Panel Bin Set 31

Primer Focus® Kit . . . . . . . . . . . . . . . . . . . . 32

Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

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Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

4SNaPshot® Kit 49

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Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . 52

557

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iv GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

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GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide v

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

Applied Biosystems Data Collection

SNaPshot Kit

GeneMapper

•GeneMapper® Software v4.0 Quick Installation and Administration Guide

(PN 4363080) GeneMapper v4.0

•Microsoft

®

Winows

®

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•

0 [Enter]

•

•

File Open Spot Set

View Filter View All Runs

Applied Biosystems 2

vi GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

Control Console

Oracle

ID

1 96 1 Sample Entry

GeneMapper® Software v4.0 Installation and Administration Guide (PN 4363080)

GeneMapper® Software v4.0 Installation and

Administration Guide (PN 4363080)

GeneMapper® Software 4.0

Installation and Administration Guide (PN 4363080)

•GeneMapper® Software v4.0 Installation and Administration Guide – GeneMapper

v4.0

•GeneMapper® Software v4.0 Getting Started Guide – GeneMapper

5

Applied Biosystems Data

Collection LOH AFLP®

SNaPshot® SNPlex

GeneMapper

•GeneMapper® Software v4.0 Online Help – GeneMapper

[F1]

Help Contents and Index GeneMapper

3

•GeneMapper® Software v4.0 Quick Reference Guide –

GeneMapper

•GeneMapper® Software v4.0 Reference and Troubleshooting Guide –

PDF

GeneMapper v4.0 Documentation CD

vii

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide vii

GeneMapper

[F1] Help Contents and Index GeneMapper

3

Applied Biosystems

jptechsupport@appliedbiosystems.com

http://www.appliedbiosystems.co.jp Customer Support

•FAQ

•

• Applied Biosystems MSDS Certification of

Analysis

•PDF

•

Applied Biosystems

viii GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

1

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 1

■SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

■SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

■GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

■ . . . . . . . . . . . . . . . . . . 7

■GeneMapper v3.7 . . . . . . . . . . . . . . . . . 7

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1

SNaPshot® Kit

2GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

SNaPshot® Kit

SNP SNP (Single Nucleotide Polymorphism) DNA

4

SNaPshot® Kit SNaPshot® Muliplex Kit

SNaPshot Multiplex Kit SNP PCR

10 SNP

SNP

ddNTP

4 ddNTP

SNP

GeneMapper

( 1-1 )

SNaPshot Multiplex Kit 10

SNP

1-2

...TCGTTGTAGCGCTTAGA...

...AGCAACATCGCTAATCT...

...TCGTTGTAACGCTTAGA...

...AGCAACATTGCTAATCT...

G-C A-T SNP

2

1

SNaPshot® Kit

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 3

1-1 SNaPshot® Muliplex Kit

1-2

Primer Focus® Kit

Primer Focus Kit SNP 4

4 ddNTP

SNaPshot 3′

GR1770

Applied Biosystems

GeneMapper®

TCGTTGTA

...NNNNNAGCAACATCGCTAANNNNN...

TCGTTGTAG

...NNNNNAGCAACATCGCTAANNNNN...

ddGTP

ddCTP

ddUTP

ddATP

5′3′

TCGTTGTAG

=

SNP

TCG

AGC

1

SNaPshot® Kit

4GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

GeneMapper Primer Focus Kit

•SNP

• Auto Panel SNP Bin

SNaPshot® Kit Applied Biosystems Kit

• SNaPshot Multiplex Kit (PN: 4323159 4323161 4323163)

• Primer Focus Kit (PN: 4329538)

SNaPshot Applied Biosystems

GeneMapper® Software v4.0 Quick Reference Guide (PN 4362816)

G

C

U

A

ddGTP

ddCTP

ddUTP

ddATP

Primer Focus® Kit

4 ddNTP

SNP

1

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 5

3 .fsa

6 Primer Focus Kit

<drive>:\AppliedBiosystems\GeneMapper\Example Data\SNaPshot

GeneMapper®

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SNaPshot Multiplex Kit GeneScan™ 120 LIZ®

ABI PRISM® 3100

Primer Focus Kit

GeneScan™ 120 LIZ® ABI PRISM® 3100

SNaPshot Kit 6

2 Marker Bin

3GeneMapper Primer Focus Kit Auto Panel

Marker Bin

1

SNaPshot® Kit

6GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

SNaPshot® Kit

GeneMapper® SNaPshot

SNaPshot® Kit

(4)

1.

2.

Table Settings

(5)

1.

2.

Panel Bin Set

(2)

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2. Ta bl e

Settings

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4. Kit Panel Marker Bin Set

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Settings

3.

4. Kit Panel Marker Bin Set

Bin

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GeneMapper®

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 7

GeneMapper®

GeneMapper

1. Start All Program Applied Biosystems GeneMapper GeneMapper

4.0

2. Login to GeneMapper

a.

b. OK

SNaPshot Kit

GeneMapper®

Software Online Help

GeneMapper v3.7

SNaPshot Kit

Analysis Method Size Standard Panel

GeneMapper

Bin/ Marker bp Bin

Marker

Bin Set/

Bin Bin Set

Marker/ SNP Marker bp

Panel/ Marker GeneMapper Panel Bin Set

Marker Bin

Kit/

1

8GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

GeneMapper® SNaPshot Kit

GeneMapper

SNaPshot Kit 2 3

4SNaPshot Kit

GeneMapper

Data Collection

SNaPshot Kit

GeneMapper®

Software v4.0 Quick Installation and Administration Guide (PN 4363080)

GeneMapper

SNaPshot

Kit 2 3 4

GeneMapper

GeneMapper®

Software v4.0 Quick Installation and Administration Guide (PN 4363080)

2

GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 9

Panel Bin Set

■ . . . . . . . . . . . . . . . 10

■ Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

■Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Marker Bin

Primer

Focus Kit Auto Panel Marker Bin

3

2 Panel Bin Set

10 GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide

GeneMapper

1. File New Project

2. New Project SNaPshot OK

GeneMapper v3.7 3

3. GeneMapper File Add Samples to

Project

4. Add Samples to Project Files

<drive>:\AppliedBiosystems\GeneMapper\Example Data\SNaPshot

GeneMapper®

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Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

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