2. Thermo Fisher Scientific
  3. GeneMapper Software

Thermo Fisher Scientific GeneMapper Software クイックスタートガイド

  • こんにちは！Applied Biosystems GeneMapper Software v4.0とSNaPshot Kitを用いたSNP解析に関するユーザーガイドの内容を理解しています。このガイドでは、データの解析方法からPanelやBin Setの作成、トラブルシューティングまで網羅されています。何かご質問があればお気軽にお尋ねください！
  • SNaPshot Multiplex KitとPrimer Focus Kitの違いは何ですか？
    GeneMapperソフトウェアのバージョン3.7を使用できますか？
    サンプルファイルはどこにありますか？
GeneMapper® v4.0
SNaPshot
®
Kit SNP
Getting Started Guide
Panel
Bin Set
Primer Focus® Kit
Panel
Bin Set
SNaPshot® Kit
GeneMapper® v4.0
SNaPshot
®
Kit SNP
Getting Started Guide
Panel Bin Set
Primer Focus® Kit
Panel
Bin Set
SNaPshot® Kit
ii GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
Copyright
© Copyright 2005, Applied Biosystems.All rights reserved.
Applied Biosystems
Applied Biosystems
:
Applera Corporation
GeneMapper Software has not undergone specific developmental validation for human identification applications. Human identification laboratories
analyzing single-source or parentage samples which choose to use GeneMapper Software for data analysis should perform their own developmental
validation studies.
:
ABI PRISM, Applied Biosystems, GeneMapper, LIZ, Primer Focus, and SNaPshot are registered trademarks, and the AB Design, Applera, GeneMapper and
SNPlex are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries.
This product includes software developed by the Apache Software Foundation
(http://www.apache.org/). Copyright © 1999-2000 The Apache Software Foundation. All rights reserved.
This product includes software developed by the ExoLab Project (http://www. exolab.org/). Copyright 2000 © Intalio Inc. Allrights reserved.
JNIRegistry is Copyright © 1997 Timothy Gerard Endres, ICE Engineering, Inc., http://www.trustice.com.
AFLP is a registered trademark of Keygene N.V.
Microsoft is registered trademark of Microsoft Corporation.
Oracle is a registered trademark of Oracle Corporation.
All other trademarks are the sole property of their respective owners.
Applera Corporation is committed to providing the world's leading technology and information for life scientists. Applera Corporation consists of the
Applied Biosystems and Celera Genomics businesses.
Part Number 4363078 Rev. B
06/2005
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide iii
v
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11
SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
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SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
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GeneMapper v3.7 . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
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2 Panel Bin Set 9
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Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
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Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3Primer Focus® Kit Panel Bin Set 31
Primer Focus® Kit . . . . . . . . . . . . . . . . . . . . 32
Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
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Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
4SNaPshot® Kit 49
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Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . 52
557
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iv GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
663
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GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide v
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
Applied Biosystems Data Collection
SNaPshot Kit
GeneMapper
GeneMapper
•GeneMapper® Software v4.0 Quick Installation and Administration Guide
(PN 4363080) GeneMapper v4.0
•Microsoft
®
Winows
®
XP
•
0 [Enter]
•
•
File Open Spot Set
View Filter View All Runs
Applied Biosystems 2
vi GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
Control Console
Oracle
ID
1 96 1 Sample Entry
GeneMapper® Software v4.0 Installation and Administration Guide (PN 4363080)
GeneMapper® Software v4.0 Installation and
Administration Guide (PN 4363080)
GeneMapper® Software 4.0
Installation and Administration Guide (PN 4363080)
•GeneMapper® Software v4.0 Installation and Administration Guide – GeneMapper
v4.0
•GeneMapper® Software v4.0 Getting Started Guide – GeneMapper
5
Applied Biosystems Data
Collection LOH AFLP®
SNaPshot® SNPlex
GeneMapper
•GeneMapper® Software v4.0 Online Help – GeneMapper
[F1]
Help Contents and Index GeneMapper
3
•GeneMapper® Software v4.0 Quick Reference Guide –
GeneMapper
•GeneMapper® Software v4.0 Reference and Troubleshooting Guide –
PDF
GeneMapper v4.0 Documentation CD
vii
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide vii
GeneMapper
[F1] Help Contents and Index GeneMapper
3
Applied Biosystems
jptechsupport@appliedbiosystems.com
http://www.appliedbiosystems.co.jp Customer Support
•FAQ
•
• Applied Biosystems MSDS Certification of
Analysis
•PDF
•
•
Applied Biosystems
viii GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
1
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 1
■SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
■SNaPshot® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
■GeneMapper® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
■ . . . . . . . . . . . . . . . . . . 7
■GeneMapper v3.7 . . . . . . . . . . . . . . . . . 7
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1
SNaPshot® Kit
2GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
SNaPshot® Kit
SNP SNP (Single Nucleotide Polymorphism) DNA
4
SNaPshot® Kit SNaPshot® Muliplex Kit
SNaPshot Multiplex Kit SNP PCR
10 SNP
SNP
ddNTP
4 ddNTP
SNP
GeneMapper
( 1-1 )
SNaPshot Multiplex Kit 10
SNP
SNP
1-2
...TCGTTGTAGCGCTTAGA...
...AGCAACATCGCTAATCT...
...TCGTTGTAACGCTTAGA...
...AGCAACATTGCTAATCT...
G-C A-T SNP
2
1
SNaPshot® Kit
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 3
1-1 SNaPshot® Muliplex Kit
1-2
Primer Focus® Kit
Primer Focus Kit SNP 4
4 ddNTP
SNaPshot 3′
GR1770
Applied Biosystems
GeneMapper®
TCGTTGTA
...NNNNNAGCAACATCGCTAANNNNN...
TCGTTGTAG
...NNNNNAGCAACATCGCTAANNNNN...
ddGTP
ddCTP
ddUTP
ddATP
5′3′
TCGTTGTAG
=
SNP
TCG
AGC
1
SNaPshot® Kit
4GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
GeneMapper Primer Focus Kit
•SNP
• Auto Panel SNP Bin
SNaPshot® Kit Applied Biosystems Kit
• SNaPshot Multiplex Kit (PN: 4323159 4323161 4323163)
• Primer Focus Kit (PN: 4329538)
SNaPshot Applied Biosystems
GeneMapper® Software v4.0 Quick Reference Guide (PN 4362816)
G
C
U
A
ddGTP
ddCTP
ddUTP
ddATP
Primer Focus® Kit
4 ddNTP
SNP
1
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 5
3 .fsa
6 Primer Focus Kit
<drive>:\AppliedBiosystems\GeneMapper\Example Data\SNaPshot
GeneMapper®
D
SNaPshot Multiplex Kit GeneScan™ 120 LIZ®
ABI PRISM® 3100
Primer Focus Kit
GeneScan™ 120 LIZ® ABI PRISM® 3100
SNaPshot Kit 6
2 Marker Bin
3GeneMapper Primer Focus Kit Auto Panel
Marker Bin
1
SNaPshot® Kit
6GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
SNaPshot® Kit
GeneMapper® SNaPshot
SNaPshot® Kit
(4)
1.
2.
Table Settings
(5)
1.
2.
Panel Bin Set
(2)
1.
2. Ta bl e
Settings
3.
4. Kit Panel Marker Bin Set
Bin
(6)
•
•
Primer Focus® Kit Panel
Bin Set
(3)
1. Primer Focus Kit
2. Tabl e
Settings
3.
4. Kit Panel Marker Bin Set
Bin
1
GeneMapper®
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 7
GeneMapper®
GeneMapper
1. Start All Program Applied Biosystems GeneMapper GeneMapper
4.0
2. Login to GeneMapper
a.
b. OK
SNaPshot Kit
GeneMapper®
Software Online Help
GeneMapper v3.7
GeneMapper v3.7
GeneMapper v3.7
GeneMapper v3.7
SNaPshot Kit
Analysis Method Size Standard Panel
GeneMapper
Bin/ Marker bp Bin
Marker
Bin Set/
Bin Bin Set
Marker/ SNP Marker bp
Panel/ Marker GeneMapper Panel Bin Set
Marker Bin
Kit/
1
8GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
GeneMapper® SNaPshot Kit
GeneMapper
SNaPshot Kit 2 3
4SNaPshot Kit
GeneMapper
Data Collection
SNaPshot Kit
GeneMapper®
Software v4.0 Quick Installation and Administration Guide (PN 4363080)
GeneMapper
SNaPshot
Kit 2 3 4
GeneMapper
GeneMapper®
Software v4.0 Quick Installation and Administration Guide (PN 4363080)
2
GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide 9
Panel Bin Set
■ . . . . . . . . . . . . . . . 10
■ Table Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
■. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
■Kit Panel Marker Bin Set Bin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Marker Bin
Primer
Focus Kit Auto Panel Marker Bin
3
2 Panel Bin Set
10 GeneMapper® Software Version 4.0 SNaPshot Kit SNP Getting Started Guide
GeneMapper
1. File New Project
2. New Project SNaPshot OK
GeneMapper v3.7 3
3. GeneMapper File Add Samples to
Project
4. Add Samples to Project Files
<drive>:\AppliedBiosystems\GeneMapper\Example Data\SNaPshot
GeneMapper®
D
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