®
Sep, 2006
®
®
2
•
•
• DNA
• Applied Biosystems 7300 7500 Real-Time PCR
Rapid Finder™
–
–
–
–
–
• Applied Biosystems 7900 HT FAST Real-Time PCR
– PCR
–
–
–
–
3
®
®
®
4
TaqMan® Pathogen Detection kit
PCR
TaqMan® Probe PCR
PCR DNA1 10 TaqMan® Pathogen Detection Kit
25g CFU
TaqMan® Pathogen Detection kit 100
IPC
TaqMan® Pathogen Detection kit IPC IPC PCR
PCR
IPC
TaqMan® Pathogen Detection kit
• 10x Target Assay Mix -20
• Negative Control 2x Environmental Master Mix 2 8
•
5
®
®
®
®
®
®
®
®
®
®
100
100
100
100
100
100
100
1
1
100
4368733
4368735
4368734
4368721
4370500
4370568
4318930
4318925
4318924
4322547
4370199
4370200
4370202
6
DNA
PrepMan® Ultra Sample Preparation Reagent
PrepMan® Ultra Sample Preparation Reagent Protocol
16,000 3
100 l PrepMan® Ultra Sample Preparation Reagent
95-100 10
16,000 3
50 l 4 1
10 l RNase free 90 l
PCR DNA
7
•Rapid Finder
–
–
–
–
–
–
– , ,
1. Rapid Finder
2.
Applied Biosystems 7300 7500 Real-Time PCR
8
1. [Create/Edit a Plate]
2. [Create New Plate] [Next]
• Template Use Existing Plate Template
3. [Plate Name]
• [Setup Operator] [Comment]
4. [Next]
9
5. • 3
•
6. [Next]
7.
8. [Next]
10
9.
10. [Next]
11.
12.
13. [Next]
Back
13
11
1. [Pipette Plate] [Close]
2. [Next]
3. Plate Name
4.
5. [Next]
6.
7. [Next]
12
8.
9.
10. [Next]
11.
12.
13. [Next]
10xTAM 2xEMM
EMM TAM
1 5
13
14.
15.
16. [Next]
17. DNA
18. [Next]
19.
Next
14
20.
24.
•
21.
22. [Next]
23.
Next
15
24.
25.
26. [Next]
27.
28. [Next]
16
1. [Run plate]
2.
3. [Next]
17
4. PCR
5.
6. [start]
7. Close
18
1.
2. [View Results]
3. Plate Name [Results by Well]
[ ] [ ] [ ] [ ]
Appendix
4. [View in SDS] !!
4
3
19
5. [Report]
20
PCR
1.
2. ( ) ( )
a. X
b. ( )
c.
[ 1 3
1 ]
(*) 10%
3.
4. 18uL
a.
b.
c. 1
d. 1
e.
[ ]
79.218.0
66.015.0
13.23.0
UNKN2
B
POS
CTRL
NEG
CTRL
NEG
CTRL
UNKN2UNKN1C
H
F
POS
CTRL
NEG
CTRL
NEG
CTRL
UNKN2UNKN1G
D
POS
CTRL
NEG
CTRL
NEG
CTRL
UNKN1E
POS
CTRL
NEG
CTRL
NEG
CTRL
UNKN2UNKN1A
121110987654321
Rapid Finder™
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