Thermo Fisher Scientific TaqMan Pathogen リファレンスガイド

®

Sep, 2006

®

2

•

• DNA

• Applied Biosystems 7300 7500 Real-Time PCR

Rapid Finder™

–

• Applied Biosystems 7900 HT FAST Real-Time PCR

– PCR

–

3

®

4

TaqMan® Pathogen Detection kit

PCR

TaqMan® Probe PCR

PCR DNA1 10 TaqMan® Pathogen Detection Kit

25g CFU

TaqMan® Pathogen Detection kit 100

IPC

TaqMan® Pathogen Detection kit IPC IPC PCR

PCR

IPC

TaqMan® Pathogen Detection kit

• 10x Target Assay Mix -20

• Negative Control 2x Environmental Master Mix 2 8

•

5

®

100

1

100

4368733

4368735

4368734

4368721

4370500

4370568

4318930

4318925

4318924

4322547

4370199

4370200

4370202

6

DNA

PrepMan® Ultra Sample Preparation Reagent

PrepMan® Ultra Sample Preparation Reagent Protocol

16,000 3

100 l PrepMan® Ultra Sample Preparation Reagent

95-100 10

16,000 3

50 l 4 1

10 l RNase free 90 l

PCR DNA

7

•Rapid Finder

–

– , ,

1. Rapid Finder

2.

Applied Biosystems 7300 7500 Real-Time PCR

8

1. [Create/Edit a Plate]

2. [Create New Plate] [Next]

• Template Use Existing Plate Template

3. [Plate Name]

• [Setup Operator] [Comment]

4. [Next]

9

5. • 3

•

6. [Next]

7.

8. [Next]

10

9.

10. [Next]

11.

12.

13. [Next]

Back

13

11

1. [Pipette Plate] [Close]

2. [Next]

3. Plate Name

4.

5. [Next]

6.

7. [Next]

12

8.

9.

10. [Next]

11.

12.

13. [Next]

10xTAM 2xEMM

EMM TAM

1 5

13

14.

15.

16. [Next]

17. DNA

18. [Next]

19.

14

20.

24.

•

21.

22. [Next]

23.

15

24.

25.

26. [Next]

27.

28. [Next]

16

1. [Run plate]

2.

3. [Next]

17

4. PCR

5.

6. [start]

7. Close

18

1.

2. [View Results]

3. Plate Name [Results by Well]

[ ] [ ] [ ] [ ]

Appendix

4. [View in SDS] !!

4

3

19

5. [Report]

20

PCR

1.

2. ( ) ( )

a. X

b. ( )

c.

[ 1 3

1 ]

(*) 10%

3.

4. 18uL

a.

b.

c. 1

d. 1

e.

[ ]

79.218.0

66.015.0

13.23.0

UNKN2

B

POS

CTRL

NEG

CTRL

NEG

CTRL

UNKN2UNKN1C

H

F

POS

CTRL

NEG

CTRL

NEG

CTRL

UNKN2UNKN1G

D

POS

CTRL

NEG

CTRL

NEG

CTRL

UNKN1E

POS

CTRL

NEG

CTRL

NEG

CTRL

UNKN2UNKN1A

121110987654321

Rapid Finder™

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Thermo Fisher Scientific TaqMan Pathogen リファレンスガイド

Thermo Fisher Scientific TaqMan Pathogen リファレンスガイド

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