Thermo Fisher Scientific TaqMan Universal Master Mix II リファレンスガイド

ブランド
Thermo Fisher Scientific
モデル
TaqMan Universal Master Mix II
タイプ
リファレンスガイド
Quick Reference Card
TaqMan
® Universal Master Mix II
TaqMan
®
Universal Master Mix II Protocol (PN 4428173)
MSDS
TaqMan
®
Universal Master Mix II (PCR) (Primer probe template
Water ) Mix TaqMan
®
Universal Master Mix II TaqMan
®
assays
PCR TaqMan
®
Universal Master Mix II Protocol
(PN 4428173)
1
a. TaqMan
®
Assay
b. TaqMan
®
Assay TaqMan
®
Universal
Master Mix II
4
2
a. 1.5- 2ml
110%
3
PCR
(µL)
PCR
50-µL .
20-µL .
TaqMan
®
Universal Master Mix II, with/no UNG, 2
25.0
10.0
1
TaqMan
®
Assay, 20
2.5
1.0
1
cDNA or DNA template + RNase-free water
‡
22.5
9.0
1 100 ng
Total Volume
50.0
20.0
—
1 100 ng cDNA RNase-free water
b.
c.
a. 96 PCR 50µL
384 PCR 20µL
b.
c.
d. PCR
! 2
4
TaqMan
®
Gene Expression Assays Quick Reference Card
5
a.
UNG
Polymerase
PCR
(40 )
HOLD
HOLD
Denature
Anneal/Extend
( )
50
95
95
60
(mm : ss)
2:00
10:00
00:15 1:00
(µL) 50 20
Uracil-N-Glycosylase (UNG) UNG
b.
6
Base line Threshold line
C
T
Ordering Information
TaqMan® Universal Master Mix II (with UNG no UNG) 2
TaqMan® Universal Master Mix II, TaqMan® reagent assays
www.appliedbiosystems.com
50-µL
Mini-Pack
1 × 1-mL tube
40
4440042
1-Pack
1 × 5-mL bottle
200
4440038
2-Pack
2 × 5-mL bottles
400
4440044
5-Pack
5 × 5-mL bottles
1000
4440045
10-Pack
10 × 5-mL bottles
2000
4440046
TaqMan
®
Universal Master Mix II, with UNG
Includes:
• AmpliTaq Gold
®
DNA Polymerase, UP (UltraPure)
• Uracil-N-Glycosylase (UNG)
• dNTPs with dUTP
• Passive Reference 1
• Optimized buffer components
Bulk-Pack
1 × 50-mL bottle
2000
4440039
Mini-Pack 1 × 1-mL tube 40 4440043
1-Pack
1 × 5-mL bottle 200
4440040
2-Pack 2 × 5-mL bottles 400 4440047
5-Pack 5 × 5-mL bottles 1000 4440048
10-Pack 10 × 5-mL bottles 2000 4440049
TaqMan
®
Universal Master Mix II, without UNG
Includes:
• AmpliTaq Gold
®
DNA Polymerase, UP (UltraPure)
• dNTPs with dUTP
• Passive Reference 1
• Optimized buffer components
Bulk-Pack 1 × 50-mL bottle 2000 4440041
For Research Use Only. Not for use in diagnostic procedures.
NOTICE TO PURCHASER: PLEASE REFER TO THE TAQMAN
®
UNIVERSAL MASTER MIX II PROTOCOL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION.
© 2009, Life Technologies Corporation. All rights reserved. Trademarks of Life Technologies Corporation and its affiliated companies: AB
®
(Design), Applied Biosystems
®
. TaqMan
and AmpliTaq Gold are registered trademarks of Roche Molecular s, Inc. All other trademarks are the sole property of their respective owners.
07/2009
www.appliedbiosystems.com Part Number 4428174 Rev. A
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Thermo Fisher Scientific TaqMan Universal Master Mix II リファレンスガイド

ブランド
Thermo Fisher Scientific
モデル
TaqMan Universal Master Mix II
タイプ
リファレンスガイド
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