James DeRose
Leica M ic r os y stems AG
Adam Clie
Leica Microsystems
Heinrich Buergers*
Leica Microsystems AG
* heinrich.buergers@leica-microsystems.com
From Eye to Insight
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CO
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CO
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Leica Microsystems (
) Leica M50 M60 M80 CMO (
) [11]
>
2 Leica M80 30.7 mm ( ) FOV
Leica M60 36.5 mm ( ) FOV ( ) Leica M60
FOV Leica M80
0.5x 0.63x
Leica M60 (FOV)
( )
4
> (FN) 23 mm (FOV)
/
(OF)
Leica M80 FOV M50 M60 ( 3 2)
> Leica M80 M60 M50 ( NA 0.15)
( [NA] 0.206) -
> FOV Leica M50 M60
M80 [12,13]
> Leica M50 M60 M80 Leica LED2000 LED2500
3 ( ) Leica M60
M80
4 Leica M80
CO2 (1)
CO
2
(2)
2
1
> ( Leica ErgoWedge)
( )
> LED
( T. Hummel)
5
( )
( )
CO
2
>
> ( ) ( 5)
Leica LED3000 (SLI)
5 ( )
B. Reversade IMB A*STAR
Greenough CMO ( )
Greenough
> CMO
>
Greenough
>
> ( )
( 6 6)
CMO
Greenough [14,15]
>
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6 Greenough CMO
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6 A) C) Greenough 3D
B) D) CMO ( )
A Greenough B CMO
C
D
Greenough
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CMO
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7
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[17,18]
[16] (GFP) (RFP) (CFP)
(YFP)
( GFP)
Leica Microsystems
Leica M165 FC M205 FA MZ10 F ( 7 )
Leica M165 FC M205 FA MZ10 F
>
(S/N)
>
Leica M205 FA ( NA 0.349) M165 FC (
NA 0.3) MZ10 F ( NA 0.25)
>
Leica M165 FC M205 FA MZ10 F
>
Leica M165 FC M205 FA
( )
>
Leica M205 FA FusionOptics [19] 3D
>
Leica M205 FA
Z
>
( Leica ErgoTubes)
7 Leica M205
FA
RFP (
)
( )
8
[20]
Leica M80
M125
( ) ( 8 )
8 Leica M125 3
RFP ( ) Leica
L. Geid T. Hummel
/
Leica M205 FA
M165 FC M205 A M205 C M165 FC M165 C
Leica DMS1000 DMS300
Leica Application Suite X (LAS X) (EDOF) Z 3D Leica
LAS X
[21] ( Leica TCS SP8 SPE (
) DM6 ( ))
Leica M205 FA
Leica M205 FA FusionOptics [19]
( )
9
[1,2] ( )
[7]
( )
>
>
>
(
)
Laura Geid Thomas Hummel
IMB A*STAR Bruno Reversade
( )
10
Leica Microsystems (Switzerland) Ltd. · Max-Schmidheiny-Strasse 201· 9435 Heerbrugg, Switzerland
+41 71 726 34 34 · +41 71 726 34 44
www.leica-microsystems.com
© 2015 Leica Microsystems (Schweiz) AG
LEICA Leica Microsystems IR GmbH FusionOptics Leica Microsystems (Schweiz) AG
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G. Manning, A quick and simple introduction to
Drosophila melanogaster, Drosophila
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Thomas H. Morgan – Biographical, The Nobel Prize in Physiology or Medicine 1933, The Nobel Foundation, Stockholm, Sweden
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Maryland, USA
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Maryland, USA
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Genome, Talking Glossary of Genetic Terms, National Human Genome Research Institute (NHGRI), National Institute of Health (NIH), Bethesda,
Maryland, USA
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L.T. Reiter, L. Potocki, S. Chien, M. Gribskov, E. Bier, A Systematic Analysis of Human Disease-Associated Gene Sequences In
Drosophila melanogaster
,
Genome Res., vol. 11, iss. 6 (June, 2001) pp. 1114–1125; doi: 10.1101/gr.169101
8.
J. Herrero, How to get all the orthologous genes between two species, Ensembl Project, Cambridge, UK
9.
F. Ho, How to Prepare Your Specimen for Immunouorescence Microscopy, Science Lab
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Phenotype, Talking Glossary of Genetic Terms, National Human Genome Research Institute (NHGRI), National Institute of Health (NIH), Bethesda,
Maryland, USA
11.
D. Goeggel, The History of Stereo Microscopy – Part III: The 19th Century – Breakthrough of Modern Microscope Manufacturing, Science Lab
12.
D. Goeggel, The History of Stereo Microscopy – Part II: The 18th Century – Greater Demands are Placed on Optics, Science Lab
13.
Objective Classes, Leica Microsystems
14.
R. Rottermann, P. Bauer, How Sharp Images Are Formed: Depth of Field in Microscopy, Science Lab
15.
D. Goeggel, Factors to Consider When Selecting a Stereo Microscope, Science Lab
16.
Genotype, Talking Glossary of Genetic Terms, National Human Genome Research Institute (NHGRI), National Institute of Health (NIH), Bethesda,
Maryland, USA
17.
C. Greb, Fluorescent Proteins – Introduction and Photo Spectral Characteristics, Science Lab
18.
Sharyn A. Endow, Detecting Gene Expression in
Drosophila
, Application Notes, Leica Biosystems
19.
D. Goeggel, A. Schué, D. Kiper, FusionOptics – Combines high resolution and depth of eld for ideal 3D optical images, Science Lab
20.
I. Maimon, L. Gilboa, Dissection and Staining of
Drosophila
Larval Ovaries, Jove, doi: 10.3791/2537
21.
J. De Bock, Mosaic Images: Bringing Together What Belongs Together, Science Lab
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